ABSTRACT
Background: Yeasts are microorganisms that have the ability to absorb aflatoxins
Objectives: The effect of the yeast Saccharomyces cerevisiae [PTCC 5177] on aflatoxin B] detoxification and absorption of toxin in in vitro [the cow rumen] was investigated
Methods: For this purpose, the yeast used in various treatments [live-treated, autoclave, heat-treated, treated with acid 100 °C] was prepared and added to the rumen of cattle. Af-latoxin E in different doses [0, 5, 10,20] ppb in the rumen were added and were incubated at 37°C for one and two hours. The amount of toxin residues was measured by ELISA using Europroxima kits. RESULTS: The results showed that microorganisms that have been treated in an autoclave have the highest amount of toxin removal [90.5%] [p<0.05]. Also, with increases in the incubation time, the amount of toxin absorbed significantly increased [78%] [p<0.05] and with increasing concentrations of toxin in vitro the yeast's ability to absorb toxin increases. These results demonstrate that the major toxin is absorbed by the yeast cell wall and therefore non-living microorganisms shown an ability to absorb higher. This is because the composition of the yeast cell wall mannoprotein that are effective at absorb in toxin
Conclusions: As a strategy for the animal feed industry the use of glycomannan yeast cell wall can be useful for reducing aflatoxin B